Peer-reviewed veterinary case report
Rapid detection of duck hepatitis virus type-1 by reverse transcription loop-mediated isothermal amplification.
- Journal:
- Journal of virological methods
- Year:
- 2012
- Authors:
- Song, Cuiping et al.
- Affiliation:
- Shanghai Veterinary Research Institute · China
- Species:
- bird
Abstract
A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of duck hepatitis virus type-1 (DHV-1) was established. Using primers specific to the highly conserved 3D gene of DHV-1, the developed RT-LAMP assay detected the viral RNA of DHV-1 extracted from both allantoic fluid and liver samples of infected ducks. The assay is as sensitive as RT-PCR, and shows no cross-reaction with other common avian viral and bacterial pathogens. In addition to detection via ethidium bromide staining following gel electrophoresis, naked-eye observation after staining with SYBR Green I dye can be used to detect RT-LAMP products; this enables field application of this assay. The findings demonstrate that RT-LAMP can serve as a helpful tool for the detection and surveillance of DHV-1 in the poultry industry.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/22465103/