Peer-reviewed veterinary case report
Rapid detection of Infectious bursal disease virus by reverse transcription loop-mediated isothermal amplification assay.
- Journal:
- Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
- Year:
- 2009
- Authors:
- Xue, Chunyi et al.
- Affiliation:
- School of Life Sciences · China
- Species:
- bird
Abstract
A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid identification of Infectious bursal disease virus (IBDV). The RT-LAMP assay used a set of 4 primers to amplify the viral protein 2 gene of IBDV for the detection of IBDV, showing not only high efficiency but also analytic specificity. The data demonstrated that the RT-LAMP assay detected 30 different IBDV isolates, had no cross-reaction with 3 other avian viruses (Infectious bronchitis virus, Newcastle disease virus, and Avian influenza virus), and obtained a 95.45% sensitivity in 22 positive clinical samples in reference to virus isolation. Therefore, this rapid, specific, sensitive, and convenient RT-LAMP assay could be applicable to the identification of IBDV in less-equipped laboratories as well as in the field.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/19901286/