Peer-reviewed veterinary case report
Protocol for modeling blood flow recovery and angiogenesis in response to hindlimb ischemia.
- Journal:
- STAR protocols
- Year:
- 2025
- Authors:
- Sharma, Sheila et al.
- Affiliation:
- Ocean State Research Institute · United States
- Species:
- rodent
Abstract
Here, we present a protocol that models peripheral artery disease using the surgical induction of hindlimb ischemia (HLI) in mice. We list the steps to surgically excise the femoral artery and assess blood flow recovery through laser Doppler imaging. We then detail procedures for visualizing immune cell recruitment and blood vessel growth to explore the role of inflammatory macrophages in angio/arteriogenesis. For complete details on the use and execution of this protocol, please refer to Mantsounga et al..
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/40333192/