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Peer-reviewed veterinary case report

Measurement of Revascularization in the Hind Limb After Experimental Ischemia in Mice.

Journal:
Methods in molecular biology (Clifton, N.J.)
Year:
2022
Authors:
Bhalla, Sohni Ria et al.
Affiliation:
School of Medicine · United Kingdom
Species:
rodent

Abstract

Peripheral vascular disease is a major cause of morbidity and mortality, and is a consequence of impaired blood flow to the limbs. This arises due to the inability of the tissue to develop sufficiently functional collateral vessel circulation to overcome occluded arteries, or microvascular impairment. The mouse hind limb model of hind limb ischemia can be used to investigate the impact of different treatment modalities, behavioral changes, or genetic knockout. Here we described the model in detail, providing examples of adverse events, and details of ex vivo analysis of blood vessel density.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/35099732/