Peer-reviewed veterinary case report
PATHOGENICITY AND IMMUNOGENICITY FOR SOME RABBIT HEMORRHAGIC DISEASE VIRUS(RHDV) STRAINS PROPAGATED IN ISOLATED HEPATOCYTES OF RABBIT
- Journal:
- Taiwan Veterinary Journal
- Year:
- 2021
- Authors:
- Pyon, Yong-Mi et al.
- Affiliation:
- Veterinary and Husbandry Faculty, Pyongyang Agricultural University, Pyongyang, Democratic People’s Republic of Korea · South Korea
- Species:
- rabbit
Plain-English summary
In this study, researchers looked at how different strains of rabbit hemorrhagic disease virus (RHDV) affected liver cells taken from healthy rabbits of various ages. They found that the virus grew better in liver cells from rabbits older than 12 weeks. When they infected non-immune rabbits with these virus strains, all rabbits infected with RHDV-1 and RHDV-3 died within two days, while one rabbit infected with RHDV-2 died within three days. Additionally, when they vaccinated rabbits with inactivated versions of the virus, the RHDV-1 and RHDV-3 strains produced a strong immune response, but RHDV-2 was less effective. Overall, the study showed that some strains of RHDV are more deadly than others, especially in younger rabbits.
Abstract
The hepatocytes were isolated from the liver of different week-age of healthy rabbits by a single two-stage pass perfusion method and suspended in Hanks’ balanced salt solution (Ca[Formula: see text]-, Mg[Formula: see text]-free). The field strains of RHDV were inoculated into the suspension and incubated for 2 days at 37∘C. After incubation, the HA titer of each strain of RHDV in supernatant of virus growth was measured using human erythrocyte O type. There were differences in the HA titers of RHDV strains according to the hepatocyte of different week-age of rabbits, the number of hepatocyte, the strain of virus (RHDV-1, RHDV-2, RHDV-3) and the duration of incubation. The HA titer was highest when the virus was cultured in the hepatocytes isolated from more than 12 week-aged rabbits. When non-immune rabbits were inoculated with the strains of virus (RHDV-1, RHDV-2, RHDV-3). respectively, all rabbits (3 heads of rabbit per group) infected with RHDV-1 and RHDV-3 died of the disease within 48[Formula: see text]h, while only one rabbit infected with RHDV-2 died within 72[Formula: see text]h. When immunized rabbits by inactivated virus strains (RHDV-1, RHDV-2 and RHDV-3), 2 strains, RHDV-1 and RHDV-3, showed the same level of HI titers with inactivated homogenate, but RHDV-2 did lower the level of titer than the homogenate.
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Search related cases →Original publication: https://doi.org/10.1142/s1682648522500044