CHARACTERISTICS FOR SOME RABBIT HEMORRHAGIC DISEASE VIRUS (RHDV) STRAINS PROPAGATED IN ISOLATED RENAL CELL OF RABBIT

Abstract

The renal cells were isolated from the kidney of different week-age of healthy rabbits by a single two-stage pass perfusion method and suspended in Hanks’ balanced salt solution ([Formula: see text]-, [Formula: see text]-free). The field strains of RHDV were inoculated into the suspension and incubated for 4 days at 37∘C. After incubation, the HA titer of each strain of RHDV in supernatant of virus growth was measured using human erythrocyte O type. There were differences in the HA titers of RHDV strains according to the renal cell of different week-age of rabbits, the number of hepatocyte, the strain of virus (RHDV-1, RHDV-2, RHDV-3) and the duration of incubation. The HA titer was highest when the virus was cultured in the renal cells isolated from more than 12 week-aged rabbits. And the HA titer of RHDV propagated in isolated renal cell was higher than hepatocytes. When immunized rabbits by inactivated virus strains (RHDV-1, RHDV-2 and RHDV-3), two strains, RHDV-1 and RHDV-3, showed the same level of HI titers with inactivated homogenate, but RHDV-2 did lower level of titer than the homogenate.