Peer-reviewed veterinary case report
Methods to generate genetically engineered mouse models of soft tissue sarcoma.
- Journal:
- Methods in molecular biology (Clifton, N.J.)
- Year:
- 2015
- Authors:
- Dodd, Rebecca D et al.
- Affiliation:
- Duke University Medical Center · United States
- Species:
- rodent
Abstract
We discuss the generation of primary soft tissue sarcomas in mice using the Cre-loxP system to activate conditional mutations in oncogenic Kras and the tumor suppressor p53 (LSL-Kras(G12D/+); p53(flox/flox)). Sarcomas can be generated either by adenoviral delivery of Cre recombinase, activation of transgenic Cre recombinase with tamoxifen, or through transplantation of isolated satellite cells with Cre activation in vitro. Various applications of these models are discussed, including anticancer therapies, metastasis, in vivo imaging, and genetic requirements for tumorigenesis.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/25636474/