Peer-reviewed veterinary case report
Immunodiagnosis of foot-and-mouth disease using mutated recombinant 3ABC polyprotein in a competitive ELISA.
- Journal:
- Journal of virological methods
- Year:
- 2012
- Authors:
- Sharma, Gaurav K et al.
- Affiliation:
- Project Directorate on Foot and Mouth Disease · India
Plain-English summary
This study focused on improving the way we can tell if livestock animals have been infected with foot-and-mouth disease (FMD) or if they have just been vaccinated against it. Researchers developed a new test using a specific part of the FMD virus called the 3ABC polyprotein, which helps differentiate between infected and vaccinated animals. They created a competitive ELISA test that showed high accuracy, correctly identifying 86.9% of infected animals and 97% of vaccinated ones. This test can be used for various types of livestock and wild animals, making it a valuable tool for controlling FMD through vaccination. Overall, the new test works well and could help manage FMD outbreaks more effectively.
Abstract
Differentiation of infected from vaccinated animals (DIVA) is essential for effective control of foot-and-mouth disease (FMD) by vaccination. The antibody response against FMD viral non-structural proteins (NSPs) has been used widely for this purpose. Among all the NSPs, the 3ABC polyprotein has been recognized as the most appropriate indicator for DIVA. In this study, mutated full-length 3ABC polyprotein was expressed in a prokaryotic system and monoclonal antibody against the recombinant protein was developed. A competitive ELISA (C-ELISA) for DIVA was standardized for different species of livestock animals using recombinant 3ABC and monoclonal antibodies. The diagnostic sensitivity and specificity of the assay were estimated by testing a panel of known serum samples consisting of sera from naive, vaccinated and infected animals as 86.9% with 66.4-97.2 (95%) confidence interval and 97% with 89.6-99.6 (95%) confidence interval respectively at 40% inhibition cut-off. The assay was validated further by testing sera from different livestock species collected at random from different parts of the country. The assay will provide a common method for testing sera from different species of livestock and wild animals. The C-ELISA is a sensitive and specific DIVA assay for FMD and can be used as a method for FMD control programme with vaccination.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/22683829/