Peer-reviewed veterinary case report
Development of an inactivated 3C(pro)-3ABC (mu3ABC) ELISA to differentiate cattle infected with foot and mouth disease virus from vaccinated cattle.
- Journal:
- Journal of virological methods
- Year:
- 2013
- Authors:
- Srisombundit, Vasinee et al.
- Affiliation:
- Faculty of Veterinary Medicine
Plain-English summary
Foot and mouth disease is a serious and contagious illness that affects animals with cloven hooves, like cows, and is still a problem in many parts of the world. Vaccinated cattle can sometimes still get infected without showing symptoms, which makes it hard to tell if an animal is sick or just vaccinated. Researchers developed a new test called the mu3ABC ELISA that can accurately tell the difference between cattle that are infected and those that have only been vaccinated. This test showed a high level of accuracy, meaning it can effectively help in controlling the disease and preventing its spread to healthy areas. Overall, the new test works well and could be a valuable tool in managing foot and mouth disease.
Abstract
Foot and mouth disease, a highly contagious disease of cloven-hoofed animals, is still endemic in Asia, Africa, and a few countries in South America. Subclinical and persistent infections usually occur in vaccinated cattle exposed to FMDV. Successful control and eradication measures need a diagnostic assay that can distinguish between immune responses to infection and vaccination. The non-structural 3ABC ELISA is the most reliable differential diagnostic assay. However, expression of the native 3ABC gene in insect cells yielded truncated versions of the proteins; thus, a monoclonal antibody to capture digested proteins is needed to develop the assay. The purpose of this study was to develop a simple indirect 3ABC ELISA using complete 3ABC protein. The full-length mutated 3ABC protein with inactive 3C(pro) (mu3ABC) gene was constructed. The histidine-tagged mu3ABC protein was produced in insect cells for easy purification and measuring. This permits simple assay design and reproducible assay development. mu3ABC ELISA had diagnostic specificity and sensitivity of 96.6% and 84%, respectively, compared to Ceditest(®) FMDV-NS. Agreement of both assays was excellent with κ value of 0.823 (p<0.05). The mu3ABC ELISA could distinguish infected from vaccinated animals. These factors are necessary for the successful development of an in-house NSP-based ELISA. Availability of a reliable assay with acceptable costs would facilitate successful disease control and the establishment of disease-free zones. Expansion of such zones may ultimately decrease the risk of introducing FMDV into disease-free countries, thus accelerating global FMD control.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/23305815/