Peer-reviewed veterinary case report
Development and evaluation of the rVP-ELISA for detection of antibodies against porcine parvovirus.
- Journal:
- Journal of virological methods
- Year:
- 2014
- Authors:
- Kong, Miaomiao et al.
- Affiliation:
- Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences · China
Plain-English summary
Researchers developed a new test called rVP-ELISA to detect antibodies against porcine parvovirus (PPV) in pigs. They created a version of a protein from the virus that closely resembles the natural one, which allowed them to set up this test. They tested 596 pig blood samples and found that about 87% were positive for PPV antibodies. When compared to another existing test, the new method showed a very high agreement rate of 96.7%. Overall, the rVP-ELISA is an effective and reliable way to identify PPV antibodies in pigs.
Abstract
The gene encoding the VP2 protein of porcine parvovirus (PPV) was expressed in an insect-baculovirus system. The recombinant (r) VP2 was similar antigenically/functionally to the native capsid protein as demonstrated by hemagglutination (HA), Western blotting using PPV positive sera. The purified rVP2 proteins were used as coating antigen to establish a rVP-ELISA method for detection of PPV positive and negative sera from pigs. The optimal operating conditions of the rVP-ELISA were: the concentration of rVP2 proteins coated on the wells was 2 μg/mL; the diluted concentration of serum was 1: 150 and that of the enzyme-labeled antibody was 1: 6000. A total of 596 sera were detected by this assay, and the average positive rate was 87%. Compared with France LSI kit, the result showed that the coincidence rate was 96.7%. In conclusion, the rVP2-ELISA is a sensitive and specific method for detecting antibodies against PPV.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/24945904/