Peer-reviewed veterinary case report
Recombinant ELISA using baculovirus-expressed VP2 for detection of antibodies against canine parvovirus.
- Journal:
- Journal of virological methods
- Year:
- 2012
- Authors:
- Elia, Gabriella et al.
- Affiliation:
- Department of Veterinary Public Health · Italy
- Species:
- dog
Abstract
The gene encoding the VP2 protein of canine parvovirus type 2 was expressed in an insect-baculovirus system. The recombinant (r) VP2 was similar antigenically/functionally to the native capsid protein as demonstrated by hemagglutination, Western blotting and hemagglutination inhibition test, using Canine parvovirus type-2 (CPV-2) positive sera. An enzyme-linked immunosorbent assay (ELISA) using the rVP2 was used for testing CPV-2 positive and negative sera from dogs and for determining the threshold of maternally derived antibodies interfering with successful vaccination of pups against CPV-2.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/22664182/