Peer-reviewed veterinary case report
An endovascular canine stroke model: middle cerebral artery occlusion with autologous clots followed by ipsilateral internal carotid artery blockade.
- Journal:
- Laboratory investigation; a journal of technical methods and pathology
- Year:
- 2013
- Authors:
- Zu, Qing-Quan et al.
- Affiliation:
- Department of Radiology · China
- Species:
- dog
Plain-English summary
Researchers developed a new model to study strokes in dogs, specifically using 15 healthy adult beagles. They created a stroke by blocking a major artery in the brain and injecting clots, then monitored the dogs for a week. They used imaging to measure the damage in the brain and found that most of the dogs (12 out of 15) survived without complications. The results showed that the model was effective for studying strokes and understanding how they affect the brain. Overall, this method was found to be reliable and could help in developing new treatments for strokes.
Abstract
Stroke is one of the leading causes of death worldwide and the main reason for long-term disability. An appropriate animal model of stroke is urgently required for understanding the exact pathophysiological mechanism of stroke and testing any new therapeutic regimen. Our work aimed to establish a canine stroke model occluding the middle cerebral artery (MCA) and blocking the ipsilateral internal carotid artery (ICA), and to assess the infarct lesions by magnetic resonance imaging. The stroke model was generated by injecting two autologous clots into each MCA, followed by 2-h ipsilateral ICA blockade (ilICAB) using a catheter in 15 healthy adult beagles. Outcome measurements included 24-h and 7-day postocclusion T2-weighted imaging (T2WI)-based infarct volume calculation. In addition, pial collateral score, canine neurobehavioral score and histopathologic results were documented. Out of 15 dogs, 12 with successful MCA occlusion (MCAO) and ilICAB survived 7 days without complications or casualties and MCA were reperfused at 7 days after occlusion. High signal intensity in the basal ganglia and cerebral cortex on T2WI was initially observed in each dog at 6 h after the procedure. The mean percentage hemispherical infarct volume corrected for edema in all dogs on T2WI at 24 h after occlusion was 12.99±1.57%, and the degree of variability was 12.08%. The infarct volumes at 24 h after occlusion correlated with pial collateral scores and canine neurobehavioral scores well. This canine stroke model with combined MCAO and ilICAB reported here were proven to be highly feasible and reproducible.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/23648562/