Peer-reviewed veterinary case report
Using Multiphoton Intravital Microscopy to Study Neutrophil Transmigration and Blood-Brain Barrier Permeability in a Mouse Model of Herpes Simplex Virus Encephalitis.
- Journal:
- Methods in molecular biology (Clifton, N.J.)
- Year:
- 2024
- Authors:
- Bricio-Moreno, Laura et al.
- Affiliation:
- Massachusetts General Hospital and Harvard Medical School · United States
- Species:
- rodent
Abstract
Multiphoton intravital microscopy (MP-IVM) is an imaging technique used for the observation of living organisms at a microscopic resolution. The tissue of interest is exposed through a window allowing imaging of cells in real time. Using MP-IVM, the temporospatial kinetics of leukocyte transendothelial migration can be visualized and quantitated using reporter mice and cell-specific fluorophore-conjugated monoclonal antibodies to track the leukocytes within and outside of vascular beds. Here we describe a method used to study neutrophil transendothelial migration and blood-brain barrier permeability in a mouse model of herpes simplex virus I (HSV) encephalitis.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/39147969/