Peer-reviewed veterinary case report
Use of the Serial Analysis of Gene Expression (SAGE) method in veterinary research: A concrete application in the study of the bovine trypanotolerance genetic control.
- Journal:
- Annals of the New York Academy of Sciences
- Year:
- 2004
- Authors:
- Maillard, Jean-Charles et al.
- Affiliation:
- c/o National Institute for Animal Husbandry (NIAH)
Plain-English summary
Researchers are using a new method called Serial Analysis of Gene Expression (SAGE) to study how certain genes in cows help them resist a disease caused by a parasite called Trypanosoma congolense. They looked at the genetic material from a specific breed of cow known for its ability to tolerate this infection, called the N'Dama. By comparing the genes that were active during the infection, they hope to identify which genes play a role in this disease resistance. This information could help improve breeding programs and develop better treatments in the future. The study is still in the early stages, but the findings could lead to important advancements in veterinary medicine.
Abstract
New postgenomic biotechnologies, such as transcriptome analyses, are now able to characterize the full complement of genes involved in the expression of specific biological functions. One of these is the Serial Analysis of Gene Expression (SAGE) technique, which consists of the construction of transcripts libraries for a quantitative analysis of the entire gene(s) expressed or inactivated at a particular step of cellular activation. Bioinformatic comparisons in the bovine genomic databases allow the identification of several up- and downregulated genes, expressed sequence tags, and unknown functional genes directly involved in the genetic control of the studied biological mechanism. We present and discuss the preliminary results in comparing the expressed genes in two total mRNA transcripts libraries obtained during an experimental Trypanosoma congolense infection in one trypanotolerant N'Dama animal cow. Knowing all the functional genes involved in the trypanotolerance control will permit validation of some results obtained with the quantitative trait locus approach, to set up specific microarrays sets for further metabolic and pharmacological studies, and to design field marker-assisted selection by introgression programs.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/15604489/