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Peer-reviewed veterinary case report

Use of RNA interference to prevent lethal murine west nile virus infection.

Journal:
The Journal of infectious diseases
Year:
2005
Authors:
Bai, Fengwei et al.
Affiliation:
Department of Internal Medicine · United States
Species:
rodent

Abstract

West Nile virus causes fatal encephalitis in humans, mice, and other vertebrates. In the present study, we demonstrate that small interfering RNAs (siRNAs) inhibit West Nile virus replication in vitro. Moreover, the administration of siRNAs to mice by hydrodynamic injection 24 h before challenge with an intraperitoneal inoculum of West Nile virus reduced the viral load and afforded partial protection from lethal infection. These data show the efficacy of the prophylactic use of siRNAs against a viral infection in vivo and suggest new strategies to combat West Nile virus.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/15747251/