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Peer-reviewed veterinary case report

Ultra-highly sensitive and wash-free bio-detection of H5N1 virus by immunomagnetic reduction assays.

Journal:
Journal of virological methods
Year:
2008
Authors:
Yang, S Y et al.
Affiliation:
Institute of Electro-optical Science and Technology
Species:
bird

Abstract

A platform for assaying avian influenza H5N1 viruses that involves measuring the ac immunomagnetic reduction of a magnetic reagent mixed with a detected sample is developed. The magnetic reagent contained magnetic nanoparticles coated with antibodies. To achieve an ultra-high sensitivity assay, a system utilizing a high-transition-temperature superconducting quantum interference device was used to sense the immunomagnetic reduction of the reagents. The results confirmed the ultra-high sensitivity of the immunomagnetic reduction assay on H5N1.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/18760307/