Therapeutic Role of Secondary Metabolites from Probiotic Strains for Ehrlich Solid Tumors in Mice.

Abstract

This study aimed to screen the bioactive components in Streptococcus equinus WC(SE-WC) and Limosilactobacillus reuteri GM(LR-GM) and estimate the therapeutic role in Ehrlich solid tumors (EST) mice model. Forty-four male albino EST mice were assigned into 7 groups and treated daily for 2 weeks, including the EST group, the EST mice that received SE-WCat a low or a high dose (0.5 ml *10or 0.5 ml *10 cfu), the EST mice that received LR-GMat the low or the high dose (0.5 ml *10or 0.5 ml *10 cfu), and the EST mice that received SE-WCplus LR-GMat the low or the high dose. Tumors were harvested, weighed, examined, and used for the determination of apoptosis-related gene expression. Samples of the intestine, liver, and kidney were gathered for histological examination. The GC-MS identified 24 and 36 bioactive compounds in SE-WCand LR-GM, respectively. The main compound in SE-WCwas lupeol; however, the main compound in LR-GMwas retinaldehyde. EST mice showed disturbances in Bcl-2, Bax, and p53 mRNA expression along with histological changes in the intestine, liver, and kidney. Administration of both bacterial strains reduced the tumor weight, alleviated the disturbances in the gene expression, and improved the histological structure of the intestine, liver, and kidney in a dose-dependent. Moreover, LR-GMwas more effective than SE-WCdue to its higher content of bioactive compounds. It could be concluded that these strains of probiotics are promising for the treatment of solid tumors.