The preventive effect of probioticX86 isolated from raw milk on-induced mastitis in rats.

Abstract

Mastitis is the most common and challenging disease that affects dairy animal welfare and causes huge economic loss in dairy industry globally. Conventional antibiotic treatment of mastitis raised the drug resistance and unsuccessful therapy. As an alternative approach, probiotic lactobacilli had shown multifunctional effects against diseases. Lactobacillus strains against mastitis are worth screening and evaluating. In this study, milk-derived Lactobacillus spp. from Ningxia, China were screenedand the anti-mastitis effect of a candidate strain was evaluated through a-induced rat mastitis model. The results showed thatX86 exhibited a high adhesion rate of MAC-T cells, presented the best probiotic properties, and demonstrated anti-effectsthrough comprehensive assessment. Furthermore,X86 alleviated pathological damage to the mammary gland, liver, and colon, inhibited the mRNA expression of pro-inflammatory cytokines factors IL-1β, IL-6, and TNF-in mammary gland tissue; and increased the content of intestine SCFAs in a rat mastitis model induced by. In conclusion, our results suggested thatX86 could be a promising probiotic for the prevention and treatment of-induced mastitis.