Peer-reviewed veterinary case report
The fluorescent detection of Paradiplozoon sp. (Monogenea: Diplozoidae) attachment clamps' sclerites and integumental proteins.
- Journal:
- The Onderstepoort journal of veterinary research
- Year:
- 2006
- Authors:
- Milne, S J & Avenant-Oldewage, A
- Affiliation:
- Department of Zoology
Abstract
In the genus Paradiplozoon, few hard structures are present therefore most of the taxonomic studies have focused on the unique ultrastructure of the sclerites. Alcohol-fixed specimens were transferred to BSA-saline for 5 min. before staining indefinitely with 5 microl WGA-TRX, 5 microl SYTO 9 and 5 microl of Calcofluor White M2R. Rhodamine, Fluorescein and DAPI bandpass filters on the microscope enabled selective light wavelength illumination of the three flourochromes by a mercury light source. This method provided an easy and rapid methodology to show the internal sclerites of attachment clamps. It is suitable for alcohol preserved specimens and may have additional applications in other helminth organisms.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/16958267/