The efficacy of topically applied Sappan wood (<i>Caesalpinia sappan</i> L.) ethanol extract during incision wound healing in albino rats.

Abstract

<h4>Background</h4>Medical manifestations in the form of incisions, burns, and trauma will trigger a natural wound-healing process that involves complex interactions between cells. Brazilin and other secondary metabolites found in Sappan wood have numerous bioactive qualities, including anti-inflammatory, anti-cancer, and antioxidant properties.<h4>Aim</h4>This study aimed to investigate the efficacy of Sappan wood (<i>Caesalpinia Sappan</i> L.) ethanol extract topically on the incision wound healing of albino rats.<h4>Methods</h4>Twenty male rats were randomly assigned into five groups with four replications, i.e., (C-) was treated ointment-based, (C+) was treated with 10% povidone-iodine, (T1, T2, and T3 groups) were treated with Sappan wood extract concentration for 6.5%, 15%, and 30%, respectively. The treatment was topically administered to wounded areas twice a day for 15 days. Wound healing was evaluated histologically as the following parameters collagen deposition, polymorphonuclear neutrophils (PMN), angiogenesis, and fibrosis degree using H&E staining. IL-2 level was evaluated using the enzyme-linked immunosorbent assay (ELISA) method. Wound length reduction was calculated on days 8 and 15.<h4>Results</h4>As a result, the 6.5% (T1), 15% (T2), and 30% (T3) Sappan wood extract groups were improved significantly (<i>p</i> < 0.05) compared to ointment-based (C-) and povidone-iodine (C+) groups on the collagen deposition, PMN, angiogenesis, fibrosis degree, and IL-2 level. In particular, the 6.5% (T1) Sappan wood extract group was highlighted significantly (<i>p</i> < 0.05) compared to other groups, evidenced by the improvisation of wound healing parameters and reduction of wound length on days 8 and 15.<h4>Conclusion</h4>In conclusion, a 6.5% Sappan wood extract revealed its applicability to improve incision wound healing in albino rats.