The Effects of Prostaglandin ETreatment on the Secretory Function of Mare Corpus Luteum Depends on the Site of Application: AnStudy.

Abstract

We examined the effect of prostaglandin (PG) Eon the secretory function of equine corpus luteum (CL), according to the application site: intra-CL injection vs. an intrauterine (intra-U) administration. Moreover, the effect of intra-CL injection vs. intra-U administration of both luteotropic factors: PGEand human chorionic gonadotropin (hCG) as a positive control, on CL function was additionally compared. Mares were assigned to the groups (= 6 per group): (1) an intra-CL saline injection (control); (2) an intra-CL injection of PGE(5 mg/ml); (3) an intra-CL injection of hCG (1,500 IU/ml); (4) an intra-U saline administration (control); (5) an intra-U administration of PGE(5 mg/5 ml); (6) an intra-U administration of hCG (1,500 IU/5 ml). Progesterone (P) and PGEconcentrations were measured in blood plasma samples collected at -2, -1, and 0 (pre-treatment), and at 1, 2, 3, 4, 6, 8, 10, 12, and 24 h after treatments. Moreover, effects of different doses of PGEapplication on the concentration of total PGF(PGFand its main metabolite 13,14-dihydro-15-keto-prostaglandin F- PGFM) was determined. The time point of PGE, hCG, or saline administration was defined as hour "0" of the experiment. An intra-CL injection of PGEincreased Pand PGEconcentrations between 3 and 4 h or at 3 and 12 h, respectively (< 0.05). While intra-U administration of PGEelevated Pconcentrations between 8 and 24 h, PGEwas upregulated at 1 h and between 3 and 4 h (< 0.05). An intra-CL injection of hCG increased Pconcentrations at 1, 6, and 12 h (< 0.05), while its intra-U administration enhanced Pand PGEconcentrations between 1 and 12 h or at 3 h and between 6 and 10 h, respectively (< 0.05). An application of PGE, dependently on the dose, supports equine CL function, regardless of the application site, consequently leading to differences in both Pand PGEconcentrations in blood plasma.