The effects of autologous hepatocyte transplantation on acute hepatic failure caused by 90% hepatectomy in rats.

Abstract

BACKGROUND/AIMS: This study aims to build rat acute hepatic failure models by 90% hepatectomy, perform autologous hepatocyte transplantation with the cells separated from the resected liver tissues, and observe the effectiveness of the transplantation on acute hepatic failure. METHODOLOGY: Ninety percent hepatectomy was performed on twenty male Sprague-Dawley rats. No other treatment was given in the control group, and autologous hepatocyte transplantation was given in the experimental group. The outcomes of two groups were compared. RESULTS: The survival rate of the rats of the experimental group was evidently higher than that of the control group (60% vs. 20%). The TB, ALT and AST levels of the experimental group were obviously smaller than the control group on the first postoperative day. Two days later the TB, AST and ALT levels of the control group were larger than the experimental group. Three days after the operation, the quantities of TB and ALT of the experimental group were evidently smaller than those of the control group. The quantity of AST of the experimental group was smaller than the control group. CONCLUSIONS: Autologous hepatocyte transplantation with multi-point injection of 0.6mL hepatocyte suspension into the spleen effectively eased the symptoms of AHF caused by 90% hepatectomy.