Tendon-derived progenitor cells improve healing of collagenase-induced flexor tendinitis.

Abstract

Tendinitis is a common and a performance-limiting injury in athletes. This study describes the value of intralesional tendon-derived progenitor cell (TDPC) injections in equine flexor tendinitis. Collagenase-induced tendinitis was created in both front superficial digital flexor (SDF) tendons. Four weeks later, the forelimb tendon lesions were treated with 1&#x2009;&#xd7;&#x2009;10autogenous TDPCs or saline. Tendinitis was also induced by collagenase in one hind SDF tendon, to study the survival and distribution of DiI-labeled TDPCs 1, 2, 4, and 6 weeks after injection. The remaining normal tendon was used as a "control." Twelve weeks after forelimb TDPC injections, tendons were harvested for assessment of matrix gene expression, biochemical, biomechanical, and histological characteristics. DiI-labeled TDPCs were abundant 1 week after injection but gradually declined over time and were undetectable after 6 weeks. Twelve weeks after TDPC injection, collagens I and III, COMP and tenomodulin mRNA levels were similar (p&#x2009;=&#x2009;0.3) in both TDPC and saline groups and higher (p&#x2009;<&#x2009;0.05) than normal tendon. Yield and maximal stresses of the TDPC group were significantly greater (p&#x2009;=&#x2009;0.005) than the saline group's and similar (p&#x2009;=&#x2009;0.6) to normal tendon. However, the elastic modulus of the TDPC and saline groups were not significantly different (p&#x2009;=&#x2009;0.32). Histological assessment of the repair tissues with Fourier transform-second harmonic generation imaging demonstrated that collagen alignment was significantly better (p&#x2009;=&#x2009;0.02) in TDPC group than in the saline controls. In summary, treating collagenase-induced flexor tendon lesions with TDPCs improved the tensile strength and collagen fiber alignment of the repair tissue. Study Design &#xa9; 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:2162-2171, 2016.