Supplementation ofextract contributes to the improvement of intestinal health in swine.

Abstract

INTRODUCTION: (vine tea), a high polyphenol content antioxidant plant resource, is renowned for its medicinal benefits. This study aimed to investigate the effects ofextract (AGE) on anti-inflammatory and antioxidant ability, enhancement of intestinal immunity, improvement of intestinal structure, and regulation of gut microbiota in swine. METHODS: A total of 135 weaned piglets were randomly divided into three groups: a control group, a low-dose group, and a high-dose group. Pigs were weighed and blood was collected on days 36, 85, and 154. The feed intake was recorded daily to calculate growth performance parameters. On day 154, five to six pigs in each group were randomly selected and euthanized to obtain a small intestine to investigate the effects of AGE on anti-inflammatory and antioxidant abilities and gut microbiota. RESULTS: The results showed that 500&#x2009;mg/kg AGE increased the expression of anti-inflammatory and immune cytokines (IL-10, IgG, and IgA) (&#x2009;<&#x2009;0.05,&#x2009;<&#x2009;0.01) and decreased the expression of proinflammatory cytokines (IL-1&#x3b2;) (&#x2009;<&#x2009;0.05) in serum. Additionally, 500&#x2009;mg/kg AGE enhanced the antioxidant capacity by increasing the GSH-Px, CAT, and SOD (&#x2009;<&#x2009;0.05,&#x2009;<&#x2009;0.01). DISCUSSION: A total of 500&#x2009;mg/kg AGE significantly increased the abundance of gut microbiota, enhanced the gut barrier, and modulated gut immunity. During the piglet phase, 500&#x2009;mg/kg AGE increased the relative abundance of(&#x2009;<&#x2009;0.05). During the growing-finishing phase, 500&#x2009;mg/kg AGE increased the relative abundance ofand(&#x2009;<&#x2009;0.05,&#x2009;<&#x2009;0.01). Overall, we recommended 500&#x2009;mg/kg AGE as a routine addition dose for swine to improve porcine growth performance and intestinal health.