Peer-reviewed veterinary case report
Simple and rapid method for routine screening of bovine leukemia virus by loop-mediated isothermal amplification assay.
- Journal:
- The Journal of veterinary medical science
- Year:
- 2017
- Authors:
- Okuwa, Yuka et al.
- Affiliation:
- Nanbu Livestock Hygiene Service Center · Japan
Abstract
Various techniques for screening and detection of bovine leukemia virus (BLV) were compared to ascertain a rapid and simple technique for routine examination. The performance of real-time PCR, nested PCR and loop-mediated isothermal amplification (LAMP) assays was compared using DNA extracted from whole blood instead of white blood cells (WBCs) of 23 cattle. Real-time PCR, LAMP and nested PCR detected 18, 16 and 11 BLV-positive cattle, respectively. These results suggest that LAMP using DNA from whole blood could enable rapid examination, as isolation of WBCs and electrophoresis is time-consuming and could be useful as a simple and rapid method for routine screening of BLV.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/27725415/