Serologic and molecular survey ofin Baghdad Province, Iraq.

Abstract

BACKGROUND: A prevalent contagious pathogenic parasite that can lead to major health issues is. AIM: The present study aimed to detect the parasitic immune response and the existence of genomic DNA in the blood of a-positive equine. METHODS: Thirty serum samples from horses suspected of having toxoplasmosis were collected from the Al-Rusafa neighborhood in Baghdad. To quantitatively investigate toxoplasma antibody levels in horse serum, an ELISA was used to evaluate immunoglobulin G (IgG) levels. Conventional (PCR) was used to identifyDNA. RESULTS: The blood levels of IgG immunoglobulin in toxoplasma-infected horses differed significantly (< 0.01) according to sex and age.-specific forward and reverse primers were generated using NCBI GenBank software. Toxoplasma genes were amplified using standard PCR. The proposed method can be used as a molecular diagnostic tool for detecting and comparing molecules using a ladder. CONCLUSION: The findings of this investigation were to ascertain whether thegenotype (UPRTF2 gene) is present. The size band of 443 bp DNA in the blood of toxoplasma-infected horses was confirmed using serological and molecular assessments. There were no statistically significant differences found by the Chi-square (&#x3c7;) test between the age groups or sexes of the seropositive and seronegative horses.