Review on anthelmintic resistances and its detection method on gastrointestinal nematodes of small ruminants

Abstract

Antihelmintic resistance is a genetic change in the ability of an individual parasite to survive the recommended therapeutic dose which is caused by factors like under-dosing, frequent drug exposures at short intervals, continuous use of drugs with a similar mode of action and treatment when parasites have small refugee. The objective of this review article is to fill the gap of knowledge on methods of detection against antihelmintic resistance of gastrointestinal nematodes of small ruminants by using different approaches like: In vivo (fecal egg count reduction test, controlled efficacy test), in vitro (egg hatch assay, larval paralysis, larval motility assays, larval development assay, adult development assay, larval feeding inhibition assay, biochemical assays) and molecular assays (PCR assays). Their main comparison between in vivo and in vitro tests are, in vivo tests do involve slaughtering the animals for the test, sensibility, specify, reputability and easiness to develop. The molecular and genetic probes capable of determining individual susceptibility and complimentary use of some of them along with their use on a suspect would allow for an increase of sensitivity were finally recommended.