Relationship ofTyphimurium 14028 strain and itsandmutants with gut microbiota dysbiosis in rats.

Abstract

Disruptions in gut microbiota, known as dysbiosis, have been increasingly linked to pathogenic infections, withTyphimurium being a notable contributor to these disturbances.We hypothesize that the. Typhimurium 14028 WT strain induces significant dysbiosis in the rat gut microbiota and that theandgenes play crucial roles in this process.. In this study, it was aimed at investigating the dysbiotic activity of the. Typhimurium 14028 WT strain on the rat gut microbiota and the roles ofandgenes on this activity.Changes in the rat gut microbiota were determined by examining the anal swap samples taken from the experimental groups of these animals using 16S rRNA high-throughput sequencing technology.In the experimental groups, the dominant phyla were determined to beand(<0.05). However, while the rate ofwas significantly reduced in those treated with the WT andmutants, no significant difference was observed in themutant compared to the control group (<0.05). In all experimental animals, the dominant species was determined to be, regardless of the experiment time and application. The analysis results of the samples taken on the third day from the rat groups infected with theTyphimurium 14028 WT strain (W2) presented the most striking data of this study.Through distance analysis, we demonstrated that a successfulinfection completely changes the composition of the microbiota, dramatically reduces species diversity and richness in the microbiota and encourages the growth of opportunistic pathogens.