Reference intervals for bone marrow cells in juvenile and young adult cats bone marrow cytology.

Abstract

BACKGROUND: The current bone marrow (BM) reference intervals (RI) are based on a limited number of cats. Age-related changes in BM variables might be important,possibly affecting the interpretation of the results. OBJECTIVES: Establish BM cytologic reference intervals (RIs) and association of age and sex on these findings, in healthy juvenile and young adult cats. METHODS: BM aspirates of cats deemed healthy based on history and clinical, CBC, serum chemistry findings, and negative retrovirus serology were obtained and examined cytologically. The examination included a 1000-nucleated differential cell count and cell morphologic assessment. RIs were calculated using parametric, robust, and nonparametric methods. The cytologic findings were examined for associations with sex and age. RESULTS: The study included 40 cats (females, 22 [55%]; males, 18 [45%]) with a median age of 1.5&#x2009;years (range 0.5-5). Most calculated RIs were similar to those previously reported. BM plasma cell and monocyte counts were weakly and positively correlated with age (r, .312 and .373, respectively; P&#x2009;<&#x2009;.05). Metarubricytes were higher infemales (mean, 25.1%; SD, 6.0%) than males (mean, 21.2%; SD, 6.0%; P&#x2009;<&#x2009;.05). CONCLUSIONS: The BM differential cell counts determined in this study can serve as RIs for cats aged 0.5-5&#x2009;years.