Peer-reviewed veterinary case report
Reduced Pancreatic Exocrine Function and Organellar Disarray in a Canine Model of Acute Pancreatitis.
- Journal:
- PloS one
- Year:
- 2016
- Authors:
- Jin, Yuepeng et al.
- Affiliation:
- Department of Surgery · China
- Species:
- dog
Plain-English summary
This study looked at how the pancreas works in dogs during the early stages of acute pancreatitis, which is a sudden inflammation of the pancreas. The researchers caused this condition in dogs by injecting a substance into the pancreas, leading to high levels of a specific enzyme in the blood and a significant drop in enzyme activity in the pancreatic fluid. They found that the cells in the pancreas showed signs of swelling and damage, and the structures inside the cells were also affected. Overall, the pancreas produced less fluid and fewer enzymes during this condition, which could help in developing new treatments for acute pancreatitis in dogs.
Abstract
The aim of the present study was to investigate the pancreatic exocrine function in a canine model and to analyze the changes in organelles of pancreatic acinar cells during the early stage of acute pancreatitis (AP). AP was induced by retrograde injection of 5% sodium taurocholate (0.5 ml/kg) into the main pancreatic duct of dogs. The induction of AP resulted in serum hyperamylasemia and a marked reduction of amylase activity in the pancreatic fluid (PF). The pancreatic exocrine function was markedly decreased in subjects with AP compared with the control group. After the induction of AP, histological examination showed acinar cell edema, cytoplasmic vacuolization, fibroblasts infiltration, and inflammatory cell infiltration in the interstitium. Electron micrographs after the induction of AP revealed that most of the rough endoplasmic reticulum (RER) were dilated and that some of the ribosomes were no longer located on the RER. The mitochondria were swollen, with shortened and broken cristae. The present study demonstrated, in a canine model, a reduced volume of PF secretion with decreased enzyme secretion during the early stage of AP. Injury of mitochondria and dilatation and degranulation of RER may be responsible for the reduced exocrine function in AP. Furthermore, the present model and results may be useful for researching novel therapeutic measures in AP.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/26895040/