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Peer-reviewed veterinary case report

Rat Model of Cardiotoxic Drug-Induced Cardiomyopathy.

Journal:
Methods in molecular biology (Clifton, N.J.)
Year:
2018
Authors:
Nakahara, Takehiro et al.
Affiliation:
Icahn School of Medicine at Mount Sinai · United States
Species:
rodent

Abstract

Cardiotoxicity from cancer drugs remains a clinical problem. To find reliable markers of cardiotoxicity, animal models were proposed and potential new diagnostic markers have been actively investigated using these models. Here we describe our protocols, using male Sprague-Dawley rats, for inducing cardiomyopathy by single injection of high-dose doxorubicin (5-10 mg/kg) or multiple injections (2-4 times) of low-dose doxorubicin (2.5 mg/kg) with combined single injection of trastuzumab (10 mg/kg). The cardiotoxicity is evaluated by imaging modalities (echocardiography and nuclear imaging), serum troponin levels, and histopathological analyses.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/29987823/