Peer-reviewed veterinary case report
Rapid preparation of gastrointestinal nematode eggs from faeces for PCR identification.
- Journal:
- Journal of microbiological methods
- Year:
- 2021
- Authors:
- Hedley, Nicole et al.
- Affiliation:
- Department of Chemistry and Biotechnology · Australia
Abstract
Detection of gastrointestinal nematodes (GIN) as both a qualitative and quantitative test is highly desirable. Methods such as multiplex and qPCR are capable of providing such results, but can be laborious and expensive. This paper presents a rapid, low-cost method of preparing GIN egg from faecal samples that produces DNA suitable for PCR analysis. We also describe a set of primers that are suitable for single-tube multiplex PCR.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/34062212/