Peer-reviewed veterinary case report
Quantitative real-time PCR assays for the concurrent diagnosis of infectious laryngotracheitis virus, Newcastle disease virus and avian metapneumovirus in poultry.
- Journal:
- Journal of veterinary science
- Year:
- 2022
- Authors:
- Mo, Jongseo et al.
- Affiliation:
- Department of Population Health · United States
- Species:
- bird
Abstract
Newcastle disease (ND), infectious laryngotracheitis (ILT) and avian metapneumovirus (aMPV) can be similar making it critical to quickly differentiate them. Herein, we adapted pre-existing molecular-based diagnostic assays for NDV and ILTV, and developed new assays for aMPV A and B, for use under synchronized thermocycling conditions. All assays performed equivalently with linearity over a 5 logdynamic range, a reproducible (R² > 0.99) limit of detection of ≥ 10 target copies, and amplification efficiencies between 86.8%-98.2%. Using biological specimens for NDV and ILTV showed 100% specificity. Identical amplification conditions will simplify procedures for detection in diagnostic laboratories.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/35187879/