Quantification of plaque lipids in the aortic root of ApoE-deficient mice by 3D DIXON magnetic resonance imaging in an ex vivo model.

Abstract

OBJECTIVES: To establish a dedicated protocol for the three-dimensional (3D) quantification of plaque lipids in apolipoprotein E-deficient (apoE(-/-)) mice using ex vivo MRI. METHODS: ApoE(-/-) mice were fed a high-fat diet (n&#x2009;=&#x2009;10) or normal food (n&#x2009;=&#x2009;10) for 3 months. Subsequently, a 3D FLASH MRI sequence was used to view the anatomy of the aortic root in the isolated hearts, where a 3D double-echo two-excitation pulse sequence (DIXON sequence) was used to selectively image plaque lipids. The vessel wall, lumen and plaque lipid volumes were quantified by MRI and histology for correlation analysis. RESULTS: DIXON MRI allowed visualisation and accurate quantification of plaque lipids. When comparing the vessel wall, lumen and plaque lipid sizes in the aortic root, Bland-Altman and linear regression analysis revealed a close correlation between MRI results and the histological data both on a slice-by-slice basis and of the volumetric measurements (vessel wall: r (2)&#x2009;=&#x2009;0.775, p&#x2009;<&#x2009;0.001; vessel lumen: r (2)&#x2009;=&#x2009;0.875; p&#x2009;=&#x2009;0.002; plaque lipid: r (2)&#x2009;=&#x2009;0.819, p&#x2009;=&#x2009;0.003). CONCLUSIONS: The combination of 3D FLASH and DIXON-sequence MRI permits an accurate ex vivo assessment of the investigated plaque parameters in the aortic root of mice, particularly the lipid content.