Peer-reviewed veterinary case report
Protocol to identify and sort antigen-specific B cells from Plasmodium-infected mice.
- Year:
- 2025
- Authors:
- Calôba C et al.
- Affiliation:
- Rosalind Franklin University of Medicine and Science · United States
- Species:
- rodent
Abstract
Here, we detail a protocol to identify an antigen-specific B cell population using SpyCage reagents tailored to bind MSP<sub>1-19</sub> antigen in a rodent malaria model. We describe steps for the preparation of splenic single-cell suspension, B cell enrichment, and staining to facilitate sorting of live cells for downstream applications such as single-cell RNA, V(D)J, and assay for transposase-accessible chromatin (ATAC) sequencing. For complete details on the use and execution of this protocol, please refer to Calôba et al.<sup>1</sup>.
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Search related cases →Original publication: https://europepmc.org/article/MED/41066245