Peer-reviewed veterinary case report
Protocol for KAT8 conditional knockout mice generation and its application in 4NQO-induced esophageal tumor model.
- Journal:
- STAR protocols
- Year:
- 2026
- Authors:
- Zhang, Dandan et al.
- Affiliation:
- China-US (Henan) Hormel Cancer Institute · China
- Species:
- rodent
Abstract
4-Nitroquinoline 1-oxide (4NQO), a quinoline derivative, induces tumor formation in mice following a multi-stage pattern analogous to human tumors, mimicking the natural carcinogenesis process. Here, we present a protocol for inducing esophageal squamous cell carcinoma (ESCC) in mice using 4NQO. We detail the acquisition of KAT8 esophagus-specific knockout mice, the establishment of 4NQO-induced ESCC, and the validation of the model via western blotting (WB), hematoxylin and eosin (H&E) staining, and immunohistochemistry (IHC). For complete details on the use and execution of this protocol, please refer to Zhang et al..
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/41739644/