Peer-reviewed veterinary case report
Proficiency monitoring of monoclonal antibody cocktail-based enzyme-linked immunosorbent assay for detection of allergen-specific immunoglobulin E in dogs.
- Journal:
- Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
- Year:
- 2015
- Authors:
- Lee, Kenneth W et al.
- Affiliation:
- Greer Laboratories Inc. · United Kingdom
- Species:
- dog
Plain-English summary
This study looked at how well different laboratories can test for specific allergies in dogs using a special blood test called macELISA, which measures a type of antibody called IgE that reacts to allergens. Researchers sent the same blood samples to 10 different labs, where 16 operators tested them without knowing which samples they were. The results showed that the labs produced very similar outcomes, with over 90% agreement in their findings. Overall, the study confirmed that this allergy test is reliable and can give consistent results, whether done by different people in the same lab or across different labs.
Abstract
The purpose of our study was to document the continued comparative proficiency of different laboratories that perform a monoclonal antibody-based enzyme-linked immunosorbent assay (macELISA) for detection of allergen-specific immunoglobulin (Ig)E in dogs. Replicate samples of 18 different sera pools were independently evaluated in a single blinded fashion by each of 16 different operators functioning in 10 different laboratories. The average intra-assay variance among reactive assay calibrators in all laboratories was 6.0% (range: 2.7-16.1%), while the average intralaboratory interassay variance was 7.5% (range: 3.9-10.9%). The overall interassay interlaboratory variance was consistent among laboratories and averaged 11.4% (range: 8.5-12.5%). All laboratories yielded similar profiles and magnitudes of responses for replicate unknown samples; dose response profiles observed in each of the laboratories were indistinguishable. Considering the positive or negative results, interassay interlaboratory concordance of results exceeded 90%. Correlation of optical density values between and among all laboratories was strong (r > 0.9, P < 0.001). Collectively, the results demonstrated that the macELISA for measuring allergen-specific canine IgE is reproducible, and documents that consistency of results can be achieved not only in an individual laboratory by differing operators but also among laboratories using the same monoclonal-based ELISA.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/26069227/