Obestatin Reduces Intestinal Damage in Experimental Necrotizing Enterocolitis in Newborn Rats.

Abstract

OBJECTIVE: To investigate the effect of pretreatment with obestatin (OB), an endogenous hormone also found in mother's milk, in experimental necrotizing enterocolitis (NEC). STUDY DESIGN: Pups were randomized into four groups: control, OB-control, NEC, and OB-NEC. NEC was induced by asphyxia and hypothermia in the NEC and OB-NEC groups. OB was administered to the OB-control and OB-NEC groups. Macroscopic scoring of the intestinal tract was evaluated and tissue samples were obtained for histopathological and biochemical examination on the fourth day. RESULTS: OB improved the macroscopic appearance of the gut and the clinical score during the experiment (&#x2009;&#x2009;<&#x2009;&#x2009;0.05). The rate of occurrence of NEC in the OB-NEC group was lower than the NEC group (&#x2009;&#x2009;=&#x2009;&#x2009;0.001). OB prevented necrosis and reduced the number of apoptotic cells in the OB-NEC group compared with the NEC group (&#x2009;&#x2009;=&#x2009;&#x2009;0.006). Furthermore, interleukin-6 and malondialdehyde levels in the OB-NEC group were lower than the NEC group (&#x2009;&#x2009;<&#x2009;&#x2009;0.05). CONCLUSION: OB reduced intestinal damage and prevented necrosis through anti-inflammatory and antiapoptotic effects in experimental NEC. This effect of OB should be confirmed in clinical studies. Furthermore, future research should investigate whether OB plays a role in NEC pathogenesis or NEC is associated with OB levels in the serum and in breast milk.