New burn model for developing consistent second- and third-degree burn injuries in rats.

Abstract

OBJECTIVE: This study's aim was: (1) introduce the digital drying oven as a reproducible, controllable, and accurate heating device for burn model creation. (2) Define the heating temperature appropriate for developing consistent second and third-degree burn injuries in rats. RESULTS: Burns appeared deeper with more distinct borders in groups (B) and (C) than in group (A). The stainless-steel rod at 100 &#xba;C created burn injuries of the second degree, evidenced by the sloughing of the epidermis and necrosis in the epithelium and upper part of the dermis. Heating at 150 and 200 &#xba;C created third-degree burn injuries, where necrosis involved the epidermis and dermis and extended to the subcutaneous fat and muscles. The depth of the burn wound in the group (B) (371.2&#x2009;&#xb1;&#x2009;41.3&#xa0;&#x3bc;m) and (C) (385.2&#x2009;&#xb1;&#x2009;38.0&#xa0;&#x3bc;m) was significantly deeper compared with the group (A) (178&#x2009;&#xb1;&#x2009;46.6&#xa0;&#x3bc;m) (P&#x2009;<&#x2009;0.001). The digital drying oven is a reliable, reproducible, and controllable heating device for creating burn models. The stainless-steel rod (63&#xa0;g and 8&#xa0;mm) heated at 100 and 150 &#xba;C with a contact time of 30&#xa0;s is adequate for creating consistent second and third-degree burn injuries in rats, respectively.