Neuropathogenesis of Usutu virus-associated disease in Eurasian blackbirds (Turdus merula) involves apoptosis through the extrinsic pathway.

Abstract

Usutu virus (USUV) is a zoonotic arbovirus causing systemic and neuroinvasive diseases in birds and flu-like or sporadic neuroinvasive disease in humans. USUV-associated encephalitis is morphologically well-characterized in birds, while the mechanisms of cell-damage in spontaneously infected birds remain poorly understood. For the closely related West Nile virus, apoptosis significantly contributes to the pathogenesis of othoflavivirus-induced neuropathology. This study investigates the involvement of apoptosis in USUV-associated encephalitis in Eurasian blackbirds (Turdus merula). To determine the apoptosis involvement, immunohistochemistry for cleaved-caspase-3 (CC3), TUNEL-assay, and RT-qPCR to compare mRNA expression levels of caspase 8 (extrinsic pathway) and caspase 9 (intrinsic pathway) were performed on brains of USUV-infected (n&#x2009;=&#x2009;26) and USUV-uninfected (n&#x2009;=&#x2009;16) blackbirds, as well as Africa 3 and Europe 3 USUV-lineages. The results showed a higher CC3 expression in USUV-infected (m&#x2009;=&#x2009;231,96&#x2009;&#xb1;&#x2009;117,033) compared to uninfected blackbirds (m&#x2009;=&#x2009;22,00&#x2009;&#xb1;&#x2009;13,31) (p&#x2009;<&#x2009;0.001). Similarly, higher TUNEL-positive cell counts were detected in USUV-infected (m&#x2009;=&#x2009;197,00&#x2009;&#xb1;&#x2009;83,56) compared to uninfected blackbirds (m&#x2009;=&#x2009;19,00&#x2009;&#xb1;&#x2009;16,08) (p&#x2009;<&#x2009;0.01). No significant differences were detected comparing Africa 3 and Europe 3 lineages. Additionally, both caspases were upregulated in USUV-infected blackbirds. Caspase 8 was in average 4,37&#x2009;&#xb1;&#x2009;0,89 (CI &#x3b1;&#x2009;=&#x2009;0,05) times higher in USUV-infected blackbirds (p&#x2009;<&#x2009;0.0001), while caspase 9 expression was in average 1,82&#x2009;&#xb1;&#x2009;0,89 (CI &#x3b1;&#x2009;=&#x2009;0,05) times higher in USUV-infected blackbirds (p&#x2009;<&#x2009;0.01). Although both apoptotic pathways are involved, the extrinsic pathway is notably more active in USUV-infected blackbirds, highlighting its role in USUV-associated brain damage.