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Peer-reviewed veterinary case report

NDUFS4: creation of a mouse model mimicking a Complex I disorder.

Journal:
Mitochondrion
Year:
2009
Authors:
Ingraham, Christopher A et al.
Affiliation:
Department of Biomedical Genetics · United States

Abstract

The Complex I NADH dehydrogenase-ubiquinone-FeS 4 (NDUFS4) subunit gene is involved in proper Complex I function such that the loss of NDUFS4 decreases Complex I activity resulting in mitochondrial disease. Therefore, a mouse model harboring a point mutation in the NDUFS4 gene was created. An embryonic lethal phenotype was observed in homozygous (NDUFS4(-/-)) mutant fetuses. Mitochondrial function was impaired in heterozygous animals based on oxygen consumption, and Complex I activity in NDUFS4 mouse mitochondria. Decreased Complex I activity with unaltered Complex II activity, along with an accumulation of lactate, were consistent with Complex I disorders in this mouse model.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/19460290/