Peer-reviewed veterinary case report
Multiplex polymerase chain reaction assay for the detection of minute virus of mice and mouse parvovirus infections in laboratory mice.
- Journal:
- Laboratory animals
- Year:
- 2013
- Authors:
- Wang, K W et al.
- Affiliation:
- Graduate Institute of Veterinary Medicine
- Species:
- rodent
Abstract
Mouse parvoviruses are among the most prevalent infectious pathogens in contemporary mouse colonies. To improve the efficiency of routine screening for mouse parvovirus infections, a multiplex polymerase chain reaction (PCR) assay targeting the VP gene was developed. The assay detected minute virus of mice (MVM), mouse parvovirus (MPV) and a mouse housekeeping gene (α-actin) and was able to specifically detect MVM and MPV at levels as low as 50 copies. Co-infection with the two viruses with up to 200-fold differences in viral concentrations can easily be detected. The multiplex PCR assay developed here could be a useful tool for monitoring mouse health and the viral contamination of biological materials.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/23492514/