Multidimensional integrated lung protection measures attenuate pulmonary inflammation and improve lung function by inhibiting the NF-κB signaling pathway in elderly patients with lung dysfunction.

Abstract

Elderly patients with lung dysfunction (EPLD) often experience chronic inflammation and impaired immune responses, significantly impacting their health outcomes. Currently, no specific treatment exists to prevent lung dysfunction in elderly patients. The detailed mechanism of lung dysfunction in elderly patients remains elusive, and this study aims to clarify it. General data and blood specimens were obtained from EPLD. A lung dysfunction mouse model was established by anesthetizing and ventilating BALB/c mice at a high tidal volume of 30 mL/kg. Blood samples and lung tissues were collected from all groups for further testing. HE staining, immunofluorescence, Western blot, ELISA, flow cytometry, and qRT-PCR were used to elucidate the molecular mechanisms of multidimensional integrated lung protection measures (MILPM) in lung dysfunction mice by targeting the NF-κB pathway. The results indicated that upregulation of the NF-κB signaling pathway accelerates the lung dysfunction process, while downregulation of the NF-κB signaling pathway can prevent it. Similarly, the upregulation of TNF-α, IL-6, IL-1β, and ROS levels is associated with the lung dysfunction process, whereas reducing their levels can serve as a preventative method against lung dysfunction development. Upregulation of the NF-κB pathway can accelerate the process of lung dysfunction.