Peer-reviewed veterinary case report
Molecular-phylogenetic characterization of microbial communities imbalances in the small intestine of dogs with inflammatory bowel disease.
- Journal:
- FEMS microbiology ecology
- Year:
- 2008
- Authors:
- Xenoulis, Panagiotis G et al.
- Affiliation:
- Department of Small Animal Clinical Sciences · United States
- Species:
- dog
Plain-English summary
This study looked at the differences in gut bacteria between dogs with inflammatory bowel disease (IBD) and healthy dogs. Researchers collected samples from the small intestine of ten dogs with IBD and nine healthy dogs to analyze their bacteria. They found that the dogs with IBD had fewer types of bacteria and a different mix of bacterial families compared to the healthy dogs. Specifically, the dogs with IBD had more bacteria from the Enterobacteriaceae family, while other families like Clostridiaceae and Bacteroidetes were also affected. Overall, the findings suggest that dogs with IBD have changes in their gut bacteria compared to healthy dogs.
Abstract
An association between luminal commensal bacteria and inflammatory bowel disease (IBD) has been suggested in humans, but studies investigating the intestinal microbial communities of dogs with IBD have not been published. The aim of this study was to characterize differences of the small intestinal microbial communities between dogs with IBD and healthy control dogs. Duodenal brush cytology samples were endoscopically collected from 10 dogs with IBD and nine healthy control dogs. DNA was extracted and 16S rRNA gene was amplified using universal bacterial primers. Constructed 16S rRNA gene clone libraries were compared between groups. From a total of 1240 selected clones, 156 unique 16S rRNA gene sequences were identified, belonging to six phyla: Firmicutes (53.4%), Proteobacteria (28.4%), Bacteroidetes (7.0%), Spirochaetes (5.2%), Fusobacteria (3.4%), Actinobacteria (1.1%), and Incertae sedis (1.5%). Species richness was significantly lower in the IBD group (P=0.038). Principal component analysis indicated that the small intestinal microbial communities of IBD and control dogs are composed of distinct microbial communities. The most profound difference involved enrichment of the IBD dogs with members of the Enterobacteriaceae family. However, differences involving members of other families, such as Clostridiaceae, Bacteroidetes and Spirochaetes, were also identified. In conclusion, canine IBD is associated with altered duodenal microbial communities compared with healthy controls.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/18647355/