Molecular characterization and phylogenetic analysis of an avian adeno-associated virus originating from a chicken in China.

Abstract

The use of adeno-associated viruses (AAVs) as vectors for gene delivery is well established; however, genomic information about avian adeno-associated virus (AAAV) and its use are still limited. In this study, an AAAV strain, YZ-1, was isolated from healthy chickens in China, and the complete genome was sequenced. The genomic DNA of YZ-1 is 4,684 nucleotides long, including two ORFs encoding the nonstructural proteins (Rep) and the structural proteins (Cap), and an inverted terminal repeat (ITR) forming a typical T-shaped palindromic structure at each end. YZ-1 was 95.0 and 92.2% identical to the other two reported AAAV strains, DA-1 and VR-865, respectively, at the nucleotide sequence level. In comparison to VR-865, frameshift mutations or deletions in the N-terminal region of the Rep78 protein or VP2 protein were observed in YZ-1 and DA-1. Phylogenetic analysis revealed that YZ-1, DA-1 and VR-865 could be classified into the avian group of the AAV family. This group and other AAVs of mammalian origin displayed almost equal divergence from pathogenic waterfowl parvoviruses, revealing that AAAV has no direct evolutionary relationship to them. This study therefore provides new genomic information about AAAV.