Modeling Endometriosis in Mice and Pharmacological Activity of the Drug Endoferin.

Abstract

A murine model of endometriosis was employed to evaluate the pharmacological efficacy of therapeutic compounds. Endometriosis was modeled by autotransplantation of uterine horn fragments onto the intestinal mesentery in three experimental paradigms: 1) with subcutaneous administration of estradiol; 2) a short-term protocol without estradiol; 3) a prolonged protocol without estradiol and with delayed administration of the test compound. In all animals, well-demarcated, rounded endometriotic lesions (endometriomas) were consistently observed at the implantation sites. Endoferin demonstrated its highest efficacy under the short-term, hormone-free protocol. The administration of exogenous estradiol interfered with the manifestation of the pharmacological activity of the test drug. Thus, a rapid in vivo method for assessing the therapeutic efficacy of candidate drugs was developed.