MLVA and LPS Characteristics ofIsolated from Humans and Dogs in Zhejiang, China.

Abstract

BACKGROUND: is a pathogenic bacterium that causes brucellosis in dogs, and its zoonotic potential has been increasing in recent years.is a rare source of human brucellosis in China, wherehas been the major pathogen associated with human brucellosis outbreaks. In late 2011, a case of ainfection was detected in a human patient in Zhejiang Province, China. To compare the genotypes between strains ofisolated from the patient and from dogs, a multiple-locus variable-number tandem-repeat analysis (MLVA-16) was performed. In addition, the lipopolysaccharide-synthesis-related genes were analyzed with thereference strain RM6/66. RESULTS: 32strains were divided into 26 genotypes using MLVA-16 [Hunter-Gaston Diversity Index (HGDI) = 0.976]. The HGDI indexes for various loci ranged between 0.000 and 0.865. All four Hangzhou isolates were indistinguishable using panel 1 (genotype 3) and panel 2A (genotype 28). However, these strains were distinctly different from other isolates from Beijing, Jiangsu, Liaoning, and Inner Mongolia at Bruce 09. The emergence of a humaninfection was limited to an area. Comparative analysis indicatedfrom canines and humans have no differences in lipopolysaccharide-synthesis locus. CONCLUSION: The comprehensive approaches have been used to analyze human and canineisolates, including molecular epidemiological and LPS genetic characteristics. Further detailed analysis of the whole genomic sequencing will contribute to understanding of the pathogenicity ofin humans.