Meta-analysis of Newcastle disease virus prevalence among poultry in mainland China.

Abstract

BACKGROUND: Newcastle disease is an extremely contagious and economically damaging poultry disease caused by Avian orthoavulavirus 1 [Newcastle disease virus (NDV)], a member of Paramyxoviridae. AIM: This study was conducted as a systematic review and meta-analysis aimed at assessing NDV prevalence among poultry populations in mainland China. METHODS: A total of 25 relevant epidemiological studies published between 1979 and 2023 were analyzed following the MOOSE guidelines. RESULTS: Our meta-analysis revealed an overall NDV prevalence of 2.17% (95% CI: 1.95%-2.42%) with significant heterogeneity (= 97.1%,< 0.001). Southern China exhibited the highest individual study prevalence, while southwestern regions reported the lowest (0.32%). No aggregated regional prevalence exceeded 10%, suggesting localized outbreaks. Prevalence decreased from 3.19% (before 2013) to 1.03% (post-2013 era), reflecting improved biosecurity and vaccination. RT-PCR-based studies showed lower heterogeneity (= 89.5%) compared to serological assays (= 97.1%), highlighting the challenges in comparing prevalence estimates across different diagnostic methods and underscoring the need for standardized diagnostics.emphasizing the need for standardized diagnostics. Publication Bias: Funnel plot asymmetry indicated underrepresentation of small-scale studies (= 0.003). CONCLUSION: These results highlight persistent NDV challenges in high-density poultry areas (e.g., southern China) and underscore the importance of molecular diagnostics (RT-PCR) for accurate surveillance. Given persistent hotspots in high-density poultry areas (e.g., Guangdong), targeted vaccination and region-specific biosecurity measures are critical to mitigating NDV transmission.