Peer-reviewed veterinary case report
Macrophage-targetedmRNA-lipid nanoparticles promote locomotor functional recovery after traumatic spinal cord injury in mice.
- Journal:
- Science advances
- Year:
- 2025
- Authors:
- Fu, Chunyan et al.
- Affiliation:
- Department of Orthopaedics of Sir Run Run Shaw Hospital and Department of Basic Medicine Sciences · China
- Species:
- rodent
Abstract
Traumatic spinal cord injury (SCI) causes severe central nervous system damage. M2 macrophages within the lesion are crucial for SCI recovery. Our previous research revealed that M2 macrophages transfected with magnetotactic bacteria-derivedgene can resist ferroptosis and enhance SCI recovery. To address the limitations of M2 macrophage transplantation, we developed lipid nanoparticles (LNPs) encapsulatingmRNA targeting macrophages (mRNA-PS/LNPs). The targeting efficiency and therapeutic effect of these LNPs in SCI mice were evaluated. Intravenous administration ofmRNA-PS/LNPs delivered moremRNAs to lesion-site macrophages than those in themRNA-LNP group, which resulted in enhancing motor function recovery, reducing lesion area and scar formation, and promoting neuronal survival and nerve fiber repair. These effects were nullified when macrophages were depleted. These findings suggest that macrophage-targeted delivery ofmRNA is a promising therapeutic strategy for promoting spinal cord repair and motor function recovery in patients with traumatic SCI.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/40138430/