LGG/LAC-MMT combination mitigates AFB-induced liver and intestinal injury in mice based on intestinal microbiota modulation.

Abstract

AFBinduces hepatotoxicity and enterotoxicity.(LAC) and(LGG), both belonging to LAB, have strong binding affinity for AFB. Montmorillonite (MMT) not only adsorbs AFBbut also serves as a carrier for LAB, thereby enhancing their colonization ability and prolonging their survival. Despite the unclear effects of LGG/LAC-MMT combination on AFB-induced tissue injury and intestinal microbiota disruption, this study aimed to determine whether it could effectively alleviate tissue damage from AFBexposure and enhance LAB colonization capacity in mouse intestines. Separately, LGG (2 × 10cfu/mL) and LAC (2 × 10cfu/mL) were combined with MMT (0.5 mg/kg), and the AFB-intoxicated mice were gavaged with the mixtures for 4 weeks. Findings suggested that LGG, LAC, and MMT supplementation restored oxidative stress and inflammatory caused by AFBto some degree. Furthermore, they altered the intestinal microbiota structure, enhancing the colonization ability of LABs, thereby alleviating liver and intestinal injury. The combination of LGG/LAC-MMT was more effective, especially LAC-MMT. Overall, LGG/LAC-MMT exhibits a synergistic effect and can effectively ameliorate AFB-induced tissue injury and intestinal microbiota disorder.